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Weigh the plates to determine the agar volume and gently add 1/20 of this volume of concentrated label medium (10 mg/m L Brd U in S medium supplemented with 1 mg/m L Amp) on top of the plates. Transfer aliquots of L1 animals to the Brd U feeding plates.

Note: Brd U can also be added at a later time point, when a certain developmental stage has been reached.

Leave at RT for 20 min to clear bacteria from the intestinal lumen.

O, because salt interferes with the sticking of animals to the poly-L-lysine coated slides.

Let the animals develop for 4 to 48 h in the presence of Brd U, dependent on the goal of the experiment.